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Andy Davies (Sales and Marketing Director, Prometic Bioseparations)
Location: bioLIVE Theater
Date: Wednesday, May 1
Time: 1:00pm - 1:20pm
Vault Recording: TBD
Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode ligands for product capture and polishing. In particular, their application in flow-through polishing has significantly improved process economy. However, significant optimisation of binding and elution conditions is often required to achieve the desired level of purity.
To overcome the deficiencies of individual multi-mode ligands, we have developed a diverse library of Multi-Mode Mimetic Ligands™ incorporating both ionic and hydrophobic groups, which offer significant flexibility in use. The Multi-Mode Mimetic Ligand™ Library is organised into a 12 x 8 array micro-column block and comprises a diverse array of ligands with a broad spread of charge, hydrophobic and hydrophilic functionalities. Screening in high-throughput mode, using robotic liquid handling systems or use of conventional hand-held multi-channel pipettes, allows for rapid identification of potential ligands for a specific target or impurities. Furthermore, adjustment of the starting protein fraction to suit individual ligands is not required as the diversity of the library enables the identification of binding ligands which are best-suited to the existing conditions and impurities.